| Rk | Team | 4x100 |
|---|---|---|
| 1 |
Class 1A - Top of Iowa - West Brittany Leibeg, Regan Eastvold, Makenzie Budach, Avery Eastvold |
50.02 |
| 2 |
Class 2A - Top of Iowa - West Colette Loges, Jalyn Hovenga, Emma Anderson, Chloe Hennigar |
Q 50.31 |
| 3 |
Class 1A - Top of Iowa - East Lindsey Moore, Macy Thorson, Chloe Costello, Morgan Wallin |
51.66 |
| 4 |
|
52.03 |
| 5 |
Class 1A - Top of Iowa - West Emmi Bartolo, Zoe Montag, Reagan Murphy, Abbie Capesius |
52.74 |
| 6 |
|
52.99 |
| 7 |
Class 1A - Top of Iowa - West Vanessa Nieto Mendez, Mallory Leerar, Cameron Haugland, Aubrey Madson |
Q 53.02 |
| 8 |
|
Q 53.23 |
| 9 |
Class 1A - Top of Iowa - East Jordyn Frost, Jayla Patten, Jalissa White, Ellie Eick |
53.38 |
| 10 |
Class 1A - Top of Iowa - East Alexis Rose, Claire McCandless, Cora Barnett, Laney Brinkman |
53.46 |
| 11 |
Class 2A - Top of Iowa - West Jessica Larson, Sadie Britson, Gretta Gouge, Tessa Fuentes |
53.61 |
| 12 |
Class 1A - Top of Iowa - West Chloe Hobscheidt, Kendra Huedepohl, Kaylen Morris, Jordyn Muhlenbruch |
54.65 |
| 13 |
Class 2A - Top of Iowa - East Madison Beyer, Ellyan Ryan, Payten Kruger, Leah Schwarck |
54.81 |
| 14 |
|
55.27 |
| 15 |
|
56.68 |
| 16 |
|
57.30 |
| 17 |
|
59.69 |
| 18 |
Class 1A - Top of Iowa - East Avery Patterson, Rachel Olsen, Lila Sheehan, Maggie McMichael |
1:00.66 |
Top of Iowa